Research on micropropagation of banana cv. Rajabulu (Musa AAB Group) was undertaken. On initiation stage, sucker was best used as explant during rainy season on solid medium containing 7 mg/l BAP + 3 mg/l IAA and average microshoots was 7 shoots/bottle. While inflorescence as explant was best for initiation during dry season on solid medium containing 9 mg/l BAP + 1 mg/l IAA and average microshoots was 18 shoots/bottle. On multiplication stage, sucker produced microshoot on medium containing 4.5 mg/l BAP + 1.0 mg/l kinetin (2.9 shoots). While inflorescense produced microshoot on medium containing 6.0 mg/l IAA + 2.0 mg/l BAP (2.4 shoots). Acclimatization of plantlets produced from sucker and inflorescense was best on medium of compost 75% + soil 25%. Key words: micropropagation, sucker, inflorescense, banana Rajabulu (Musa AAB Group)
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