Aktivitas Anticendawan Bacillus cereus 11UJ terhadap Rhizoctonia solani dan Pyricularia oryzae

Yadi Suryadi (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
I Made Samudra (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
Tri Puji Priyatno (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
Dwi Ningsih Susilowati (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
Puji Lestari (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
Sutoro Sutoro (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)

Jurnal Fitopatologi Indonesia
Vol 11 No 2 (2015)

Microbial secondary metabolites is an important resource for antifungal development in disease control strategy. The objective of this study was to screen Bacillus cereus 11UJ, an antagonistic rhizosphere bacteria for potential secondary metabolite production against rice fungal pathogens, i.e. Rhizoctonia solani and Pyricularia oryzae. The antagonistic effect of crude exract was evaluated using sterile filter paper discs on PDA medium. The ethyl acetate extracts of the bacterium showed a better antifungal activity to P. oryzae than those of R. solani. The inhibitory effect of the filtrate proved the potency of the isolates to produce antifungal. Analysis of pyrolysis gas chromatography-mass spectrometry showed that B. cereus 11UJ produces 3 major compounds i.e; 9,19-cyclolanostan-3-ol, acetate, (3.beta.)- (CAS) cycloartanyl acetate (13.14%); 4-(2’,2’-dimethyl-6’-methyliden-1’-cyclohexyliden)-3-methyl-2-butanone (9.72%); and stigmast-5-en-3-ol oleat (9.09%) which suggested to play an important role in the suppression of rice fungal pathogens.