Jurnal Fitopatologi Indonesia
Vol. 13 No. 3 (2017)

Deteksi dan Identifikasi Spesies Meloidogyne Penyebab Umbi Berbintil pada Kentang Asal Sulawesi Utara

Utami, Budi Sri (Unknown)
Supramana, Supramana (Unknown)
Giyanto, Giyanto (Unknown)



Article Info

Publish Date
06 Sep 2017

Abstract

Root-knot nematode (Meloidogyne spp.). is one of the main constraint of potato production in North Sulawesi.  Little is known about Meloidogyne species infecting potatoes in North Sulawesi.  Therefore, research was conducted to identify Meloidogyne spp. on potatoes in North Sulawesi and further study their relationship with related species from other countries.  Infected potato tubers with pimple-like knot symptom were collected from three potato production centers, i.e. Kakenturan (South Minahasa), Purworejo (East Bolaang Mongondow) and Singsingon (…?? Bolaang Mongondow).  Morphological identification was conducted based on the perineal pattern of  the female; whereas molecular identification was conducted by PCR using specific primer for ITS-rDNA, followed by DNA sequencing and phylogenetic analysis. Two Meloidogyne species were identified i.e.  M. javanica (samples form Kakenturan, Purworejo and Singsingon) and M. incognita (samples from Purworejo). M. javanica and M. incognita from North Sulawesi are similar to the related species from China with homology level of 97.5 % and 100 %, respectively.

Copyrights © 2017






Journal Info

Abbrev

jfiti

Publisher

Subject

Medicine & Pharmacology

Description

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and ...