Jurnal Hortikultura Indonesia (JHI)
Vol. 1 No. 2 (2010): Jurnal Hortikultura Indonesia

Kultur Jaringan Kantong Semar (Nepenthes mirabilis)

Diny Dinarti (Unknown)
Urip Sayekti (Unknown)
Yayu Alitalia (Unknown)



Article Info

Publish Date
05 Mar 2014

Abstract

ABSTRACTThis research was about in vitro propagation of Pitcher Plant Nepenthes mirabilis. The aims of this research were to determine: 1) the influence of kind and concentration of in vitro medium and also plant growth regulator on germination of Nepenthes mirabilis, 2)the effect of BAP and NAA on shoot multiplication of N. mirabilis. This research consisted of three experiments and all experiments used Completely Randomized Design. On the first experiment MS (Murashige and Skoog) and KC (Knudson C) were used, i.e. full (1), three-fourth (3/4), one-half (1/2) and one-fourth (1/4) Salt Concentration. On the second experiment 10 composition media were used, i.e. ½ MS, ½ MS+TDZ 0.01 ppm, ½ MS+IAA 0.1 ppm, ½ MS+GA3 10 ppm, ½ MS+150 ml coconut water, 1/4KC, 1/4 KC+TDZ 0.01 ppm, ¼KC+IAA 0.1 ppm, 1/4KC+GA3 10 ppm and 1/4KC+ 150 ml coconut water. On the third experiment BAP with 0, 0.5, 1, 2 ppm and NAA 0, 0.1, 0.2, 0.5 ppm were used as factor for shoot multiplication.The result showed that KC medium was the best medium for germination percentage of N. mirabilis (64%). MS or KC medium with ½ or ¼ salt concentration was the suitable for germinating time of N. mirabilis (average 39 days after showing). Adding of TDZ, IAA, and GA3 significantly increased germination percentage (70 – 90%) and decreased germinating time (27 – 38 days after showing). Culture growth on medium with BAP 0 - 1 ppm was the best medium for shoot and leave induction. The best medium for root growth of N. mirabilis was MS or KC medium without NAA.Key words: Nepenthes mirabilis, MS, KC, TDZ, IAA, GA3, BAP, NAA, in vitro propagation, medium

Copyrights © 2010






Journal Info

Abbrev

jhi

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal Hortikultura Indonesia (JHI) merupakan media untuk publikasi tulisan ilmiah dalam bahasa Indonesia maupun bahasa Inggris yang berkaitan dengan berbagai aspek dalam bidang hortikultura. Jurnal Hortikultura Indonesia (JHI) terbit tiga kali setahun (April, Agustus, dan ...