Peripheral Blood Mesenchymal Stem Cells Isolated from Indonesia Long Tailed Monkey (Macaca fascicularis)

Agus Harsoyo (Primatology Doctoral Program. Bogor Primates Research Center, Research Institutions and Community Service, Bogor Agricultural University, Indonesia)
Dondin Sajuthi (Department of Veterinary Clinic Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia)
Arief Boediono (Division of Anatomy Histology and Embryology, Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia)
Yoga Yuniadi (Divison of Arrhythmia and Device Implantation, Department of Cardiology and Vascular Medicine, Faculty of Medicine University Indonesia, National Cardiovascular Center Harapan Kita Hospital, Indonesia)
Irma H Suparto (Bogor Primate Reseach Center, Department of Chemistry, Faculty of Math and Science, Bogor Agricultural University,Indonesia)

ACTA VETERINARIA INDONESIANA
Vol. 6 No. 2 (2018): Juli 2018

An experiment to compare age of Macaca fascicularis (Mf) as pheripheral blood (PB) mesenchymal stem cell (MSC) isolate sources and the impact of its concentration on the pheriperal blood mononucleous cells (PBMC) development has been conducted. Twelve male Mf were used in this experiment. Three different age groups (infant (A1), juvenil (A2) and adult (A3)) of the Mfs were compared as treatments. Isolate of pheriperal blood MSC were created by taking 1 ml, 5 ml or 10 ml the Mfs pheriperal blood, processed them into PBMC, counted, isolated, cultured, subcultured, pelleted, extracted for their messenger Ribonucleic Acid (mRNA). Reverse transcriptase - polymerase chain reaction (RT-PCR) were conducted to obtain complentary Deoxyribonucleic Acid (cDNA). PCR amplification were performed to look cluster differentiation (CD) of the MSC gene expression. Incomplete block design was used and the data were analysed using descriptive statistic and T-Test. The results showed that PBMC counted from infant, juvenil and adult were 6.78 – 7.28, 6.18 – 7.30, and 6.01 – 7.34 log cell, respectively. The subculture and pelleting cells were only obtained from A3 with positive 73, 90, 105 and negative 34, 45 CD markers. It is concluded that pheriperal blood of adult Mf can be utilized as MSC source.