Application of Chitosan as Antibacterial for Pangasius Fillet at Low Temperature Storage

Windi Damayanti (Program Studi Perikanan Fakultas Perikanan dan Ilmu Kelautan Unpad Fakultas Perikanan dan Ilmu Kelautan Universitas Padjajaran Jalan Raya Bandung-Sumedang Km 21 Jatinangor UBR 46000 Telepon [phone] Faks. [phone])
Emma Rochima (Program Studi Perikanan Fakultas Perikanan dan Ilmu Kelautan Unpad Fakultas Perikanan dan Ilmu Kelautan Universitas Padjajaran Jalan Raya Bandung-Sumedang Km 21 Jatinangor UBR 46000 Telepon [phone] Faks. [phone])
Zahidah Hasan (Program Studi Perikanan Fakultas Perikanan dan Ilmu Kelautan Unpad Fakultas Perikanan dan Ilmu Kelautan Universitas Padjajaran Jalan Raya Bandung-Sumedang Km 21 Jatinangor UBR 46000 Telepon [phone] Faks. [phone])

Jurnal Pengolahan Hasil Perikanan Indonesia
Vol 19 No 3 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia

AbstractThis research were to obtain the optimal concentration of chitosan as an antibacterial material wich had the longest of storage period of pangasius fillet at low temperature storage (5-10oC). The method used is an experimental method with a completely randomized design, four treatments and three replications. Pangasius fillet was soaked with chitosan 0%, 1%, 2% and 3% for 3 minutes, then packed with styrofoam and plastic wrap and stored at low teperature. The observation were made on day 1st, 3rd, 5th, 6th ,7th, 9th,10th, 11th and 12th. The parameters observed total bacterial colonies, the degree of acidity (pH), weight loss, and antibacterial activity of chitosan using disc diffusion methods. The conclusion of research was that the 2% chitosan is optimal concentration for pangasius fillet storage at a low temperature until day 11th, with a total 6.7 X105 cfu/g of bacterial colonies, pH 6.67 and total 6,78 % of weight loss.Chitosan also has a higher antibacterial capability against Escherichia coli (Gram negative bacteria) than Staphylococcus aureus and Bacillus subtilis (Gram positive bacteria).