Characterization of Endogenous Transglutaminase Enzyme of Yellow Pike Conger’s Liver (Congresox talabon)

Santhy Wisuda Sidauruk (Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan Institut Pertanian Bogor)
Tati Nurhayati (Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelauta Institut Pertanian Bogor)
Untung Trimo Laksono (Program Studi Teknologi Hasil Perikanan, Jurusan Ilmu Kelautan dan Perikanan Politeknik Negeri Pontianak)

Jurnal Pengolahan Hasil Perikanan Indonesia
Vol 20 No 3 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia 20(3)

Transglutaminases have been found in various living organism, such as mammals, plants,microorganisms, and marine organisms including fishes. Transglutaminases have many various functionssuch as food or non-food application and in pharmaceticals industries. This research was aimed tocharacterize transglutaminase that obtained from fisheries byproducts such as catadromous fish the yellowpike conger’s liver.. The yield of yellow pike conger’s liver was 0.88±0.11%. Crude transglutaminase from liverof yellow pike conger had specific activity of 1.375 U/mg with optimum pH at 7.5 and optimum temperatureat 50°C. The activity of transglutaminase were increased on Mg2+ ion dependent. Crude transglutaminasehad molecular weight of 27.48; 37.00; 69.51; 78.89; 88.18; 108.45; 134.10; and 172.12 kDa.