In a survey of chrysanthemum growing fields and greenhouses in Brastagi, Sumatera Utara; Cianjur, JawaBarat; Malang, Jawa Timur; and Tabanan and Buleleng, Bali, some chrysanthemum cultivars were found showingmild leaf mottling, vein-clearing or vein banding of leaves and slight loss of flower quality. Out of 287 samplessurveyed, the disease incidence ranged between 7.50% and 67.44%. Through DAS-ELISA, the saps extractedfrom diseased plant samples, 34.84% were found to be positively reacted with serum anti-CVB, but not with seraanti- CMV, -TSWV, -PVY and -TMV. The virus isolate was then designed as CVB Indonesian isolate. The viruswas sap-transmissible to narrow host range. The virus induced systemic symptom on Nicotiana benthamiana, N.clevelandii, N. tabacum var. Burley, N. tabacum var. Havana; local necrotic lesion on Chenopodium amaranticolo,C. quinoa and both local and systemic symptom on Petunia hybrida.
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