Penentuan Kadar Protein dan Fraksi Protein Crude Antigen Cysticercus cellulosae dari Isolat Lokal

Leksana Aditya Kris Nugraha (Praktisi Dokter Hewan, Jl. Raya Sukowati Km. 2 No. 31, RT01 RW05, Gambiran, Sragen Jawa Tengah, Indonesia 57212)
Ida Bagus Ngurah Swacita (Laboratorium Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar Bali, Indonesia 80225.)
Ida Ayu Pasti Apsari (Laboratorium Parasitologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar Bali, Indonesia 80225.)

Buletin Veteriner Udayana
Vol. 11 No. 2 Agustus 2019

Cysticercosis is a parasitic disease caused by tapeworm larvae, called Cysticercus. Cysticercus found in pigs is Cysticercus cellulosae which is the larvae of the tapeworm Taenia solium. The purpose of this study was to determine the levels and fractions of Crude protein antigen C. cellulosae. C. cellulosae used is local isolates obtained from infected pigs originating from Karangasem - Bali. Determination of protein content larvae were performed using an Invitrogen Quibit Fluorometer and determination of protein fractions larvae techniques using SDS-PAGE electrophoresis. The results showed that levels of the protein C. cellulosae from local isolate was 867 µg/ml. As for protein fractionation obtained 6 bands protein with a molecular weight: bands 1 = 120.5 kDa, bands 2 = 99.8 kDa, bands 3 = 60.8 kDa, bands 4 = 47.7 kDa, bands 5 = 35.6 kDa, and bands 6 = 23.3 kDa.