Idenfitikasi Bakteri Asam Laktat Isolat 9A Asal Kolon Sapi Bali secara Fenotipik

Mita Ekamelinda (Praktisi Dokter Hewan di Kota Denpasar-Bali)
I Wayan Suardana (Laboratory of Veterinary Public Health Faculty of Veterinary Medicine Udayana University)
Komang Januartha Putra Pinatih (Laboratory of Microbiology, Faculty of Medicine, Udayana University)

Buletin Veteriner Udayana
Vol. 10 No. 2 Agustus 2018

Lactic acid bacteria are the type of bacteria that has benefits in food and health industries as a biopreservative, fermentative, or probiotics. Bali cattle are known as potential host for specific lactic acid bacteria. The aim of this study is to identify phenotypically lactic acid bacteria 9A isolated from bali cattle’s colon, that producing a substance which known has potency as antimicrobial. In this study, phenotypic identification included conventional method and API 50 CHL. The result of this study showed that lactic acid bacteria isolate 9A was Streptococcus sp., whereas identification by kit API 50 CHL showed isolate 9A as Lactobacillus fermentum with 83% identity. The difference between the results of conventional method and kit API 50 CHL, may indicate the difference in sensitivity and specificity of the two methods, hence it needs further confirmation.