This research was conducted to determine the enzyme kinetics (Km and Vmax) of endogenous polygalacturonase (PGs) isolated from cocoa pulp. This research was carried out by assaying PGs activities in various concentration of citrus pectin subtrates which were between 0,1 – 1,0% with 0,1% interval. The result showed that the enzyme kinetics of endogenous PGs from cocoa pulp (Km) was 0,37% and Vmax was 6,69 µmol of galacturonic acid/minute/ml.
Jurnal Biologi Udayana (p-ISSN 1410-5292 | e-ISSN 2599-2856 | DOI 10.24843/jbiounud) managed by the Department of Biology, Udayana University, published in two formats namely print and online regularly twice a year (June and ...