ABSTRACT---Microbe is a microscopic living organism which is closely linked to human life. One of    patogenic microorganisms such as Pseudomonas  aeruginosa  may cause diseases. Sulfure of several plants are bioactive as antimicrobial. The properties of sulfure in Allium cepa L leads to an assumption that it has antimicrobial properties. Therefore, this study involved the antimicrobial test. The determination steps of antimicrobial Allium cepa L extract consisted of the Allium cepa L extract preparation, microbial regeneration and antimicrobial  test. The antibacterial   determination were performed by paper disk method. Based on  the research  data, determination of antimicrobial white  Allium  cepa  L  extract resulted in inhibition zone area showed that the extract owned antimicrobial property.  Key words : Allium cepa L, sulfure, Pseudomonas aeruginosa
                        
                        
                        
                        
                            
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