Lactic Acid Bacteria (LAB) is a digestive tract microflora that play a positive role in poultry health. The number and diversity of LAB in the digestive tract affected by several factors, among them was the kind of feed. The purpose of this research was to know the molecular diversity of Lactic Acid Bacteria (LAB) in broiler’s ileum and cecum after feeding with prebiotic bran and Rhizopus oryzae fermented bran which was added to commercial feed. The molecular analysis was done using T-RFLP method , Hae III and Msp I were used as restriction enzymes. The number of phylotype, relative abundance, Shannon diversity index (H '), evenness (E), and Dominance (D) were examined . The results indicated that the addition of bran prebiotics on commercial feed showed a higher diversity of lactic acid bacteria on broiler’s ileum and cecum, compared with the addition of Rhizopus oryzae fermented bran. The dominant BAL types are Lactobacillus spp, L. delbrueckii subs. Bulgaricus, L. intermedius, L. amilovorus, uncultured bacteria 87 bp, 280 bp, 331 bp and unidentified bacteria 74 bp, 82 bp, 131 bp. Keywords: Diversity, Lactic Acid Bacteria, Prebiotics, Bran, T-RFLP
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