Identifying active compounds of soursop ethanolic fraction as α-glucosidase inhibitor

Eko Mugiyanto (Sekolah Tinggi Ilmu Kesehatan Muhammadiyah Pekajangan Pekalongan)
Agung Nur Cahyanta (Department of Pharmacy, STIKES Bhamada Slawi)
I Made Agus Sunadi Putra (Department of Pharmacology, Universitas Mahasaraswati)
Siswa Setyahadi (Department of Biotechnology, Indonesian Agency for the Assessment and Application of Technology)
Partomuan Simanjuntak (Department of Biotechnology, Indonesian Institute of Sciences)

Pharmaciana: Jurnal Kefarmasian
Vol 9, No 2 (2019): Pharmaciana

Postprandial hyperglycemia is triggered by two enteric proteins (α-amylase and α-glucosidase) connected to the brush border of intestinal cells. In diabetic populations, Type 2 Diabetes Mellitus is more prevalent, and α-glucosidase inhibition is the suitable therapy for delaying glucose intake after meals. This study was designed to investigate chemical compounds for their potential inhibitory effects on α-glucosidase. It employed a spectrophotometric method with p-nitrophenyl-α-D-glucopyranose from Saccharomyces cerevisiae as an alpha-glucosidase substrate. The ethanolic fraction was acquired from assay- or activity-guided fractionation. The findings showed that the ethanolic fraction of soursop leaves exhibited the most significant inhibitory activity with an IC50 of 0.17 μg/ml. The data analysis and active compounds identification were carried out by LC-MS and FT-IR.  The active compounds of the resulted mixture comprised of Muricatin C, cis-Reticulatacin-10-one, and 3-Methylquercetin 7-[galactosyl-(1->4)-glucoside].