Jurnal Ilmu dan Teknologi Kelautan Tropis
Vol. 8 No. 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis

ANALYSIS OF SECONDARY METABOLITES, ANTIBACTERIAL ACTIVITY AND COMPOUND COMPOSITION IN THE SEA CUCUMBER Bohadschia sp. EXTRACT

Abdullah Rasyid (Pusat Penelitian Oseanogafi LIPI)



Article Info

Publish Date
06 Apr 2017

Abstract

Bohadschia sp. is one of the sea cucumber species that has potential to be developed as a source of antibacterial from the sea. Samples of sea cucumber Bohadschia sp. used in this study collected from the Ratai bay waters, Lampung. This study aims to determine the type of secondary metabolites, antibacterial activity and compound composition analysis containing in the sea cucumber extract. Identification of secondary metabolites by observation of color reactions, precipitation and foam. The method used to antibacterial activity test was the agar diffusion method, while identification of the composition of compounds performed with Gas Chromatogaphy-Mass Spectroscopy (GC-MS) method.Top of FormThe results showed that the type of secondary metabolites contained in the extract of sea cucumber Bohadschia sp. were steroids and saponins. The extract of sea cucumber Bohadschia sp. showed antibacterial activity against Bacillus subtilis and Vibrio eltor. Results of GC-MS were 12 compounds and have a similarity index same or more than 90%. All compounds consist of organosilicon cyclic, fatty acid, steroid, cyclo alkene and alkena. The compound with biggest abundance was cholest-5-en-3-yl nonanoate (4.89%) and retention time was 37.370 minutes.

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Journal Info

Abbrev

jurnalikt

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Library & Information Science

Description

Aims and Scope Journal of Tropical Marine Science and Technology (Jurnal Ilmu dan Teknologi Kelautan Tropis) is a scientific journal in the field of tropical marine science and technology. We have a aims and scope to focus in publishing a good quality scientific articles for dissemination of ...