Jurnal Hutan Tropika
Vol 10 No 2 (2015): Volume 10 Nomor 2 Tahun 2015

SKRINING ENZIM DARI SAMPEL DAUN JELUTUNG RAWA (Dyera lowii Hook f.) UNTUK ANALISIS ISOZIM SEBAGAI STUDI AWAL KERAGAMAN GENETIK (Screening Enzyme from Leaf of Dyera lowii Hook f. to Isozyme Analysis. The First Study of Genetics Variety) : Tri Suwarni Wahyudiningsih

Admin JHT (Unknown)



Article Info

Publish Date
29 Aug 2019

Abstract

ABSTRACT Jelutung rawa (Dyera lowii Hook f.) is the commercial and endemic species of peat-swamp forest with sensitive status, therefore the research to analyze its genetics variety is very important. The research was aimed to analyze the seven of enzyme in the leaf of D. lowii that well-marked by the emergence of polymorphic tape pattern consistently. The research was conducted at November to December 2012 in the tree improvement laboratory of Gadjah Mada University. Leaf of D. lowii samples were gotten from D. lowii plantation at the Cilik Riwut Street, nine km from Palangka Raya City. There are seven enzyme system to screening, namely Shikimate Dehydrogenase (SHD; EC 1.1.1.25), NAD(P)H-Diaphorase (DIA; EC 1.6.2.2), Glutamate Oxaloacetate Transaminase (GOT: EC 1.11.1.7), Acid Phosphatase (ACP; EC 3.1.3.2), 6-Phosphogluconate Dehydrogenase (6-PG; EC 1.1.1.44), Esterase (EST; EC 3.1.1), dan Peroxidase (POD; EC 1.11.1.7). Stages of research were preparation, leaf extraction, supernatant, electroforesis “Wendel & Weeden”, colouring, fixation, and gel dehydration. Isozyme was detected using Rf (Relative value from Bromophenol Blue) to make the zymogram. Polymorphic tape pattern consistently was resulted by enzymes of DIA, POD, ACP, EST, and GOT, whereas polymorphic tape pattern inconsistently was resulted by enzymes SHD and 6-PG. Furthermore, the polymorphic tape pattern consistently could to analyze genetics variety. The first study of genetics variety showed that there are four locus at the leaf of D.lowii, namely Est-1, Est-2, Est-3, and Got-1 in the 14 of alel. Keywords: Dyera lowii, enzyme, isozyme analysis, polymorphic

Copyrights © 2015






Journal Info

Abbrev

JHT

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

adalah jurnal yang memiliki fokus dalam bidang ilmu dan teknologi kehutanan tropika serta semua aspek yang terkait dengan bidang ini. Jurnal ini memiliki ISSN: 1693-7643. Jurnal Hutan Tropika diikelola oleh jurusan Kehutanan, Fakultas Pertanian, Universitas Palangka Raya (Jurnal Hutan Tropika is a ...