This research aims to know the effects of olive oil to changes profil lipid (total cholesterol, LDL, HDL, and triglycerides)  in the blood of the Rattus novergicus who were given a high cholesterol diet. This research is experimental research methods laboratory with pre and post test control . Homogeneous data and normal data  next performed a test of paired t-Test and LSD test. All test data using the 95% significance level (p = 0.05). Conclusion of this research is the olive oil was able to lower the lipid profile, cholesterol 22,61%, LDL 38,48%, triglycerides 17.61% and increase the HDL 25.32%  in the blood of rats high cholesterol diet
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