Deteksi DNA Mikrofilaria Brugia malayi dengan Teknik PCR-Pockit Nucleic Acid Analyzer Pada Nyamuk di Kabupaten Pidie

Yulidar Yulidar (Balai Penelitian dan Pengembangan Kesehatan Aceh, Jalan Sultan Iskandar Muda Blang Bintang Lorong Tgk. Dilangga No. 9 Lambaro, Aceh Besar, Indonesia)
Nur Ramadhan (Balai Penelitian dan Pengembangan Kesehatan Aceh, Jalan Sultan Iskandar Muda Blang Bintang Lorong Tgk. Dilangga No. 9 Lambaro, Aceh Besar, Indonesia)
Rosdiana Rosdiana (Balai Penelitian dan Pengembangan Kesehatan Aceh, Jalan Sultan Iskandar Muda Blang Bintang Lorong Tgk. Dilangga No. 9 Lambaro, Aceh Besar, Indonesia)
Veny Wilya (Balai Penelitian dan Pengembangan Kesehatan Aceh, Jalan Sultan Iskandar Muda Blang Bintang Lorong Tgk. Dilangga No. 9 Lambaro, Aceh Besar, Indonesia)

BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA)
Volume 16 Nomor 1 Juni 2020

Filariasis is a vector-borne disease and still a public health problem in Pidie District. Transmission of filariasis infection to humans occurs through the bite of a mosquito vector carrying infective larval stage filaria (L3). This cross-sectional study conducted from February to November 2017 in Pidie district (Kambuk Payapi and Kambuk Nincah Village). Mosquitoes collection carried out during 2 periods with modified human landing collection methods. The third instar larvae infective DNA by PCR Technique-Pockit Nucleic Acid Analyzer. The results showed that the number of mosquitoes caught was 2,309 which consists of 7 genus of Culex sp., Aedes sp., Anopheles sp., Armigeres sp., Mansonia sp., Uranotaemia sp., and Verallina sp. The dominant mosquito of the Culex genus collected from the filed was Culex sitiens. The results of PCR analysis of DNA found that positive third instar larvae of Brugia malayi infective in Cx. sitiens, Cx. quiquefasciatus, Aedes vexans and Mansonia indiana. Transmission of filariasis infection in Pidie community with the discovery of the source of infection in the mosquito's body.