Jurnal Biodjati
Vol 5, No 1 (2020): May

Polymorphic Identification of Simple Sequence Repeat (SSR) Marker for Developing Aluminum-Tolerance Upland Rice

Yuliana Galih Dyan Anggraheni (Research Center for Biotechnology-LIPI
Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia, Telp. +62-21-8754587, Fax.+62-21- 8754588)

Enung Sri Mulyaningsih (Research Center for Biotechnology-LIPI
Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia, Telp. +62-21-8754587, Fax.+62-21- 8754588)

Dody Priadi (Research Center for Biotechnology-LIPI
Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia, Telp. +62-21-8754587, Fax.+62-21- 8754588)

Puspita Deswina (Research Center for Biotechnology-LIPI
Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia, Telp. +62-21-8754587, Fax.+62-21- 8754588)

Yuli Sulistyowati (Research Center for Biotechnology-LIPI
Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia, Telp. +62-21-8754587, Fax.+62-21- 8754588)

Eko Binnaryo Mei Adi (Research Center for Biotechnology-LIPI
Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia, Telp. +62-21-8754587, Fax.+62-21- 8754588)

Ambar Yuswi Perdani (Research Center for Biotechnology-LIPI
Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia, Telp. +62-21-8754587, Fax.+62-21- 8754588)

Fiqolbi Nuro (Research Center for Biotechnology-LIPI
Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia, Telp. +62-21-8754587, Fax.+62-21- 8754588)

Yashanti Berlinda Paradisa (Research Center for Biotechnology-LIPI
Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia, Telp. +62-21-8754587, Fax.+62-21- 8754588)



Article Info

Publish Date
30 May 2020

Abstract

SSR marker is one of the genetic markers widely applied in plant breeding programs. The application of molecular markers in plant breeding is meant to accelerate the selection of cross-progeny. The research aimed to identify the SSR primers polymorphism between the parent and control that linked to Al tolerance and verify the cross-progeny of five crosses. The result gained from 37 SSR primers used in this study showed that only nine primers are polymorphic. These nine polymorphic primers are RM257, RM214, RM247, RM205, RM490, RM262, RM569, RM271, and RM19. The application of polymorphic markers on five cross-progeny which have shown the same band pattern as the parents and tolerant control on the use of 9 SSR primers recorded as follows: RM257 2 lines, RM214 5 lines, RM247 5 lines, RM205 lines, RM490 13 lines, RM262 5 lines, RM569 7 lines, RM271 4 lines, and RM19 6 lines. The selected SSR primers linked to Al tolerance in this research can be used as a reference for molecular breeding strategies to develop new Al tolerance rice varieties in dryland conditions.

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