THE EFFECT OF ANTIFUNGAL ACTIVITY OF β-1,3-GLUCANASE Trichoderma reesei AGAINST ROOT FUNGI OF Ganoderma philippii

Sri Wahyuni Budiarti (Balai Pengkajian Teknologi Pertanian (BPTP) Jawa Tengah Bukit Tegalepek Ungaran Jawa Tengah 50501)
S.M. Widyastuti (Fakultas Kehutanan, Universitas Gadjah Mada, Jalan Agro No 01 Bulaksumur Yogyakarta 55281)

Widyariset
Vol 14, No 2 (2011): Widyariset

Purifi ed three isoform β-1,3-glucanases of 90 kDa β-1,3-glucanase-I, 75 kDa β-1,3-glucanase-II, and 64 kDa β-1,3-glucanase-III derived from Trichoderma reesei isolate T13were tested in vitro for its antifungal activity to the root fungi of Ganoderma philippii. Antifungal activity was tested by bioassay using a modifi ed cup plate method and microscopic observation to determine the presence of abnormal mycelia growth of G. philippii. The result showed that antifungal activity of those three isoform β-1 ,3-glucanase (β-1,3-glucanase-I, β-1,3 glucanase-II, and β-1,3-glucanase-III) in vitro could not form growth inhibition against the mycelia of G. philippii. Microscopic observation revealed that the enzyme caused necrotic lesion, whereas the combination of β-1,3-glucanase and chitinase from T. harzianum (Sigma) caused lyzed hyphae of G. philippii.