BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014

Testing Reliability of Serum Samples as a DNA Source on Captive Breeding Long-tailed Macaques (Macaca fascicularis)

Perwitasari-Farajallah, Dyah (Unknown)
Farajallah, Achmad (Unknown)

Article Info

Publish Date
28 Nov 2014

Abstract

The serum realibility was tested in captive breeding conditions on the long-tailed macaques (Macaca fascicularis). Serum and buffy coat were applied to standard protocol of DNA extration following the amplification of microsatellite DNA locus of D2S1777 by PCR and visualized PCR products by means of PAGE with silver staining.  The amount of genomic DNA extracted from serum was sufficient for genotyping individuals at DNA microsatellite locus of D2S1777 with the allele size is approximately 160 bp.  We show that serum can provide a reliable alternative for obtaining DNA where taking blood using anticoagulant is impracticable

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Journal Info
BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Website

Abbrev

biotropia

Publisher

Southeast Asian Regional Centre for Tropical Biology

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Description

BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and ...