BIOTROPIA - The Southeast Asian Journal of Tropical Biology
No. 25 (2005)

PRODUCTION AND UTILIZATION OF CELLULASE FROM TRICHODERMA VIRIDE

SURYAHADI SURYAHADI (Department of Animal Nutrition and Feed Science, Bogor Agricultural University,)
R. HIDAYAT (Department of Animal Nutrition and Feed Science, Padjadjaran University, Bandung, Indonesia.)
S. WULANDARI (Faculty of Animal Science, Jember Agricultural Poly technique, Jember, Indonesia)
K.G. WIRYAWAN (Department of Animal Nutrition and Feed Science, Bogor Agricultural University, Bogor, Indonesia.)



Article Info

Publish Date
17 Nov 2011

Abstract

  An appropriate preservation technology for forage such as silage needs to be developed in order to overcome the shortage of tropical forage during the dry season. A good quality of silage is obtained by decreasing the fibre contents (Neutral Detergent Fibre, Acid Detergent Fibre and lignin). The research was conducted in two stages: 1). Production and activity test of crude enzymes from Trichoderma viride and 2). Comparative test using crude enzymes from  Trichoderma viride strain QM 9414 (CETV) and commercial cellulase (Cellulase “Onozuka R-10”, heidelberg) on rice straw silage. The treatments were arranged as follows : P0=Untreated rice straw silage, P1=Treated with commercial cellulase at 10 IU kg-1, P2= Treated with CETV at 3.8 IU kg-1, P3=Treated with CETV at 7.6 IU kg-1  and P4= Treated with CETV at 11.4 IU kg-1 of fresh rice straw. All treatments were enriched with 5% molasses. The result of the research indicated that: 1). The activity of crude enzymes from Trichoderma viride strain QM 9414 (CETV) was 1.52 IUml-1. 2). The addition of both enzymes did not decrease dry matter of rice straw silage; however, organic matter significantly decreased (p

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Journal Info

Abbrev

biotropia

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Description

BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and ...