CHARACTERIZATION OF XYLANASE FROM A XYLANOLYTIC- THERMOPHILIC BACTERIUM ISOLATED FROM GUNUNG PANCAR HOT SPRING, WEST JAVA

ANTONIUS SUWANTO (Biotechnology Research Center, Bogor Agricultural University)
DWI SURYANTO (Dept. of Biology, Faculty of Science and Mathematics, North Sumatra University, Medan, Indonesia)
ANJA MERYANDINI (Dept. of Biology, Faculty of Science and Mathematics, Bogor Agricultural University, Bogor, Indonesia)

BIOTROPIA - The Southeast Asian Journal of Tropical Biology
No. 17 (2001)

A xylanolytic-thermophilic bacterium (IT-08) was isolated from Gunung Pancar Hot Spring after two days of enrichment in Modified Thermus Medium (MTM) supplemented with 0.5% oat spelt xylan. 16S-rRNA sequence analysis indicated that IT-08 resembles Bacillus thermoleovorans, a species of thermophilic bacteria. When grown on xylan containing media, IT-08 produces a thermoactive xylanase. Xylanase from IT-08 was active at temperatures between 40 and 100°C, at pH values between 4.0 and 9.0 with optimum values obtained at 80°C and pH 6.0, respectively. SDS-PAGE and zymogram analysis demonstrated that a crude xylanase complex of IT-08 comprised two active bands with molecular masses of 78 and 60 kDa. Keywords: xylanase / xylanolytic-thermophilic bacteria