Jurnal Natural
Vol. 15 No. 1 (2019): Jurnal Natural

Analisis Polimorfisme Nukleotida Tunggal (SNP) Daerah 3’UTR Gen LDLR Penduduk Papua

Asri Saffanah Pratiwi (Jurusan Kimia FMIPA Universitas Papua)
Achmad Taher (Jurusan Kimia FMIPA UNIPA)



Article Info

Publish Date
01 Apr 2019

Abstract

Single nucleotide polymorphism (SNP) is a single nucleotide difference in the arrangement of DNA base strands that can show genetic variation. The LDLR gene is a low density lipoprotein (LDL-R) receptor gene that functions to regulate cholesterol levels in the blood. The LDLR gene is composed of 18 exons and contains a 3’untranslated region (3’UTR) which plays an important role in regulating gene expression. This study aims to analyze the SNP in an area of 3'UTR LDLR genes from 6 University of Papua students from Papua. The research was carried out by polymerase chain reaction method to multiply the number of target DNA, then sequenced to find out the sequence of nucleotide bases. The results of this study were from 6 individuals, found 2 SNPs at position *52 and *504 with nucleotide diversity (π) of 0.00149. These polymorphisms forms 3 types of haplotypes, namely GG, GA and AA with a haplotype diversity of 0.600 ± 0.215.

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Journal Info

Abbrev

jn

Publisher

Subject

Agriculture, Biological Sciences & Forestry Chemistry Mathematics Physics

Description

Jurnal Natural merupakan jurnal ilmu-ilmu matematika dan pengetahuan alam (basic science) untuk bidang: matematika, kimia, statistika, biologi, dan fisika termasuk ilmu terapan yang terkait seperti bioteknologi, kimia pangan, keanekaragaman hayati, dan ...