Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam
Vol 1, No 2 (2014): Wisuda Oktober 2014

PRODUKSI DAN UJI AKTIVITAS SELULASE DARI ISOLAT BAKTERI SELULOLITIK SUNGAI INDRAGIRI

Kiki Apriani (Unknown)
Yuli Haryani (Unknown)
Ganis Fia Kartika (Unknown)



Article Info

Publish Date
14 Jul 2014

Abstract

Cellulolytic bacteria has been isolated from water and sediment sample of Indragiri river waters. Cellulase was produced by cellulolytic bacterial isolates in 1% Carboxy Methyl Cellulose (CMC) liquid medium at 37°C, 150 rpm for 24 h. The specific activity of cellulase was determined by Nelson-Somogy’s method (cellulase enzyme activity) and Lowry’s method (protein concentration). 914-5 sedimen isolates showed the highest activity of the enzyme 12,246.10 -4 ± 0,5885.10 -4 U/mL from 17 other isolates that was significantly different at (P≤0,05) compared with the other isolate.

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