TELKOMNIKA (Telecommunication Computing Electronics and Control)
Vol 17, No 4: August 2019

Characterization of excitation source LEDs and sensors without filters for measuring fluorescence in fluorescein and green leaf extract

Miguel Ángel Garrido Tamayo (Universidad Nacional de Colombia)
Fredy Edimer Hoyos Velasco (Universidad Nacional de Colombia)
John E. Candelo-Becerra (Universidad Nacional de Colombia)



Article Info

Publish Date
01 Aug 2019

Abstract

This paper presents the characterization of excitation source LEDs and sensors without filters for measuring fluorescence in fluorescein and green leaf extract. For this purpose, eight light-emitting diodes (LEDs) were used with the following characteristics: one blue, one green, one red, one infrared, and four violets. The first four LEDs were used as sensors without filters to detect fluorescence induced by the other four violet LEDs in 11 samples of different fluorescein concentrations and in 14 samples of different dilutions of green leaf extract. The results show that infrared LEDs can detect the red emission of green leaf extract and red and infrared LEDs detect the fluorescence of fluorescein in concentrations of up to 1.8 μM. The developed system allows and facilitates teaching optical spectroscopy in basic education without incurring high costs.

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Journal Info

Abbrev

TELKOMNIKA

Publisher

Subject

Computer Science & IT

Description

Submitted papers are evaluated by anonymous referees by single blind peer review for contribution, originality, relevance, and presentation. The Editor shall inform you of the results of the review as soon as possible, hopefully in 10 weeks. Please notice that because of the great number of ...