Wood Research Journal : Journal of Indonesian Wood Research Society
Vol 3, No 1 (2012): Wood Research Journal

Expression of Xyloglucanase (AaXEG2) in Open-field-grown Poplars

Haruka Iizuka (Department of Bioscience, Tokyo University of Agriculture Setagaya-ku, Tokyo156-8502)
Tomomi Kaku (Research Institute for Sustainable Humanosphere Kyoto University, Uji, Kyoto 611-0011)
Toru Taniguchi (Forest Bio-Research Center, Forestry and Forest Product Research Institute, Ibaraki 319-1301)
Manabu Kurita (Forest Bio-Research Center, Forestry and Forest Product Research Institute, Ibaraki 319-1301)
Ken-ichi Konagaya (Forest Bio-Research Center, Forestry and Forest Product Research Institute, Ibaraki 319-1301)
Katsuaki Ishii (Forest Bio-Research Center, Forestry and Forest Product Research Institute, Ibaraki 319-1301)
Teiji Kondo (Forest Tree Breeding Center, Forestry and Forest Product Research Institute, Ibaraki 319-1301)
Kei’ichi Baba (Research Institute for Sustainable Humanosphere Kyoto University, Uji, Kyoto 611-0011)
Rumi Kaida (Department of Bioscience, Tokyo University of Agriculture Setagaya-ku, Tokyo156-8502)
Teruaki Taji (Department of Bioscience, Tokyo University of Agriculture Setagaya-ku, Tokyo156-8502)
Yoichi Sakata (Department of Bioscience, Tokyo University of Agriculture Setagaya-ku, Tokyo156-8502)
Takahisa Hayashi (Department of Bioscience, Tokyo University of Agriculture Setagaya-ku, Tokyo156-8502)



Article Info

Publish Date
27 Aug 2017

Abstract

The transgenic expression of Aspergillus aculeatus xyloglucanase with 35S promoter, which has been maintained in greenhouse-grown transgenic poplars for 10 years, was assessed to determine their expression level in open-field-grown poplars. This level was slightly decreased in the poplars grown in fertile soil for four years, and there were proportionatel y decreased levels of xyloglucanase activity among their tissues, in which the shoots exhibited slightly lower specific activities than was observed in the leaves and roots. Xyloglucan content was greatly decreased in the walls of the tissues in the transgenic lines, although the degradation levels of xyloglucan were slightly lower for the poplars grown in fertile soil than for those grown in non-fertile soil. The results showed that the level of expression, together with xyloglucan degradation in their walls, was decreased for the poplars grown in fertile soil.

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Journal Info

Abbrev

wrj

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Wood Research Journal is the official journal of the Indonesian Wood Research Society. This journal is an international medium in exchanging, sharing and discussing the science and technology of wood. The journal publishes original manuscripts of basic and applied research of wood science and ...