METAMORFOSA Journal of Biological Sciences
Vol 7 No 2 (2020)

Isolasi Dan Identifikasi Senyawa Antijamur Daun Jeringau (Acorus calamus Linn.) Sebagai Pengendali Jamur Athelia rolfsii Sacc. Penyebab Penyakit Busuk Batang Pada Tanaman Kedelai

Susun Susun Prawanayoni (Unknown)
Sang Ketut Sudirga (Unknown)



Article Info

Publish Date
28 Sep 2020

Abstract

The study was carried out to isolate and identify active compounds in jeringau leaf extract (Acorus calamus) and test their activity to inhibit the growth of Athelia rolfsii fungus that causes stem rot in soybean plants. The study began with extraction of Jeringau leaves, extract inhibition test on Athelia rolfsii fungus, partition, fractionation with column chromatography and thin layer chromatography (TLC), fraction inhibition test and identification of active compounds by Gas Chromatography-Mass Spectroscopy (GC-MS) analysis. Coarse jeringau leaf extract can inhibit A. rolfsii fungus with inhibition zone diameter of 25 mm. The results of the analysis of active fractions of jeringau extract using GC-MS (GCMS-QP2010 Ultra SHIMADZU) obtained 24 active compounds namely: Ethanol, 2-(2-ethoxyethoxy)-CAS; 6-Okthenal,3,7-dimethyl; beta-Citronellol; Trans-Geraniol; 6-Octen-1-ol 3,7-dimethil-acetate; Benzene 1-methoxy-4-(2-propenil); 2,6-Otahen-1-ol 3,7-dimethil-acetate; Caryophyllen; 3-Allyl-6- methoxyphenol; Dodecanoic acid methil ester; Methil tetradecanoate; Cis-Asarone; Alpha hexylcinnamic aldehyde; Hexadecanoic acid methyl esteter; 7-Acethyl-6-ethyl-1-1-4-4-tetramethylhetralin; benzyl benzoate; (2(Chloro-phenyl)-(2-Methoxy-6-MI; 9-Octadecenoic acid methyl ester; 9.12-Octadecadienoic acid (Z.Z)-methyl ester; Oxinaneoctanoic acid 3-octyl-methyl ester cis:; Octadecenoic acid 9.10-didydroxyl-methyl ester; Oxinaneoctanoic acid 3-octyl-methyl trans; 9-Octadecenoic acid (Z)-methyl ester (CAS); 1.2-Benzenedicarboxylic acid, bis (2-ethylhexy). Keywords: Extract, isolation, identification, jeringau, Athelia rolfsii

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Journal Info

Abbrev

metamorfosa

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Description

METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of ...