The research wasaimed to theidentifyspecies and density of sea cucumbers at southernbeach of Bali are Mertasari beach, Segara beach, and Mengening beach. The identification ofspecies was conducted in the Laboratorium of EcologyDepartment of Biology, Faculty ofNatural Science and Mathematics, University of Udayana from December 2013 toJanuary 2014.A size of 1 x 1m quadrat squaretransect sampling method was employed in this research.Identification of sea cucumbers was performed based on their morphology and shape ofspicules. GSIwas used to observed reproductionphase of sea cucumber.Two species of seacucumbers found ware Holothuria atra and Holothuria sp. Holothuria atra was found atMertasari beach, Sanur village, southern of Denpasar and Segara beach, Tuban, Kuta, Badungwhile Holothuria sp. was faound atMengening beach. Segara beach showed the highestdensityof4.333 individual/m2,while Mertasari beach showed the lowest density 0.875individual/m2. GSI Value ofMertasari, Segara, andMengening beach showed the activationofphase ofI andII.Keywords: Holothuroidea, species, sea cucumber, density, GSI
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