One effort to the waste treatment is through the process of making fish silage. The main BAL cultures used in research adalah Lactobacillus-2 1BL plantarum and Lactobacillus plantarum 1A-2 obtained from the microbiology laboratory University School of Medicine Brawijaya. Waste tuna obtained from PT. Beeje Food Probolinggo. Treatment applied by varying the addition of lactic acid bacteria inoculum (0%, 0.1%, 0.3%, 0.5% and 1% (v / w)), either as a single inoculum (L. plantarum 1BL- 2 or L. plantarum 1A-2) and as an inoculum mixture (L. 1BL plan-indigo-2 and 1A-2) .Parameter measured as good quality silage is: dry matter (% DM), ash (%), organic matter (% BO), silage temperature (0C), the percentage of damage to silage, pH, the amount of BAL (log 10 cfu). From this study showed that the use of Lactobacillus plantarum inoculum 1A-2 and Lactobacillus plantarum 1BL-2 with different variations and concentrations provides good enough influence on the quality of silage as feed for fish. Single inoculum 1A-2 produces a lower pH (3,67- 4,18) and lactic acid content of 0.30 to 0.34 mg mL-1. In all treatments, the level of damage is quite small (<5%). Loss of dry matter, with the addition of a single inoculum provide a relatively small number of loss is 1BL-2 (0.01 to 3.97%) and 1A-2 (0.31 to 5.18%). Inoculum concentration treatments do not provide a real difference to the quality of silage, making it the smallest concentration at 0.1% v / w which is recommended to be added to the silage-making.
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