Eucheuma spinosum, red algae, contains secondary metabolites that act as antibacterial. It can be used as an active compound in the production of alcohol-free hand sanitizer. This research was aimed to determine the gram-positive and gram-negative antibacterial activity, pH, and viscosity of alcohol-free hand sanitizer containing E. spinosum extract. The results showed that the alcohol-free hand sanitizer containing 8% E. spinosum extract had characteristics in the form of a gel with the antibacterial activity of gram-positive bacteria (Bacillus subtilis) of 8.83±0.76 mm and gram-negative bacteria (Escherichia coli) of 8.67±0.58 mm, pH appropriate to pH of skin human (5.83±0.29), and viscosity following the hand sanitizer standard (2708 cps).
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