Performance of Caffeine Content Analysis in Robusta sp and Theobroma cacao L using Iodometry, UV-Vis Spectrophotometry and High Performance Liquid Chromatography

Yeanchon H. Dulanlebit (Pattimura University)
Nikmans Hattu (Chemistry Department, Faculty of Mathematics and Natural Science - Pattimura University)
Ritti P. Sangadji (Food and Drug Authority Office in Tanimbar Island Region)
Risma Mahulette (Chemistry Study Program, Faculty of Education and Teacher Training - Pattimura University)
Nataniel Kelmanutu (Chemistry Study Program, Faculty of Education and Teacher Training - Pattimura University)
Sandriliati Asari (Chemistry Study Program, Faculty of Education and Teacher Training - Pattimura University)

The Journal of Pure and Applied Chemistry Research
Vol 9, No 3 (2020): Edition September-December 2020

Caffeine is a xanthine alkaloid compound found in Robusta sp and Theobroma cacao L. The caffeine extract was obtained by evaporating CHCl3 using a rotary evaporator and determine its content using iodometry, UV-Vis spectrophotometry and High Performance Liquid Chromatography, then validated by measurement methods. The iodometric caffeine content in Theobroma cacao L is 900 mg/kg while UV-Vis spectrophotometry is 4,000 mg/kg, and for HPLC in Robusta sp is 19,475 mg/kg. The iodometry performance gives the linearity value, R2 of 0.9791. The precision values at 5.0 mg/L and 15.0 mg/L are 4.6% and 8.3%. Accuracy for 5.0; 10.0; and 15.0 mg/L are 106%, 100% and 94% respectively. The  performance of UV-Vis spectrophotometry gives  linearity value, R2 of 0.9948. The precision value at 1.0 mg/L and 6.0 mg/L gives the variance coefficient value of 4.2% and 1.2%, while the accuracy at 1.0 mg/L and 6.0 mg/L are 99% and 101%.