Journal of Aquaculture and Fish Health
Vol. 10 No. 2 (2021): JAFH Vol. 10 No. 2 June 2021

In Vitro Analysis of Antibacterial Activities of Curry Leaf (Murraya koenigii) Extract Towards Bacteria Edwardsiella tarda

Arif Syaifurrisal (Aquaculture Study Program, Aquaculture Master Program, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang 65141, Indonesia)
Arief Prajitno (Aquaculture Study Program, Aquaculture Master Program, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang 65141, Indonesia)
Mohamad Fadjar (Aquaculture Study Program, Aquaculture Master Program, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang 65141, Indonesia)
Farid Mukhtar Riyadi (Aquaculture Study Program, Aquaculture Master Program, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang 65141, Indonesia)
Annisa Isti Fauziyyah (Aquaculture Study Program, Aquaculture Master Program, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang 65141, Indonesia)



Article Info

Publish Date
28 May 2021

Abstract

This study analyzed the antibacterial activity of curry leaf extract (Murraya koenigii) on the growth of Edwardsiella tarda bacteria. This study aims to determine the bioactivity and antibacterial effectiveness of M. koenigii leaf extract against the growth of E.tarda bacteria. Inhibition test was carried out by delusion (MIC test) and diffusion (disc test) methods. MIC test used 5 variations of concentration: 1 mg/L, 10 mg/L, 100 mg/L, 500 mg/L and 1,000 mg/L on TSB (Tryptone Soya Broth) media; it was incubated for 24 hours. While the disc test used 5 variations of concentration: 100 mg/L, 200 mg/L, 300 mg/L, 400 mg/L and 500 mg/L on TSA (Tryptone Soya Agar) media and incubated for 2x24 hours. Chloramphenicol (5 mg/L)  was used as a positive control, and distilled water was used as a negative control.                    M. koenigii leaf extract contains natural bioactive; it was bacteriostatic antibacterial due to bacteria's growth after 48 hours incubation. The highest inhibition diameter of E.tarda was 7,20 mm at a concentration of 500 mg/L after 24 hours incubation. The highest inhibitory effectiveness was at a concentration of 500 mg/L with effectivity 56.3%, while it declined to 46,44% after 48 hours incubation.

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Journal Info

Abbrev

JAFH

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

The Journal of Aquaculture And Fish Health (JAFH) has an objective to publish and provide high-quality scientific contributions to the field of fisheries. These contributions came from innovative researches that encourage science and technology development in the field of fisheries and marine ...