Musamus Fisheries and Marine Journal
Volume 3 Number 2, April 2021

Genetic Diversity and Phylogenetic of Longtail Tuna (Thunnus tonggol) Landed in Pabean Fish Market, Surabaya

Ida Ayu Astarini (Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Badung, Bali-Indonesia)
Shella Ayu Ardiana (Program Studi Ilmu Kelautan, Fakultas Kelautan dan Perikanan, Universitas Udayana, Badung, Bali-Indonesia)
I Nyoman Giri Putra (Program Studi Ilmu Kelautan, Fakultas Kelautan dan Perikanan, Universitas Udayana, Badung, Bali-Indonesia)
Putu Dian Pertiwi (Yayasan Biodiversitas Indonesia, Denpasar, Bali-Indonesia)
Andrianus Sembiring (Yayasan Biodiversitas Indonesia, Denpasar, Bali-Indonesia)
Astria Yusmalinda (Yayasan Biodiversitas Indonesia, Denpasar, Bali-Indonesia)
Danie Al Malik (Yayasan Biodiversitas Indonesia, Denpasar, Bali-Indonesia)



Article Info

Publish Date
01 Mar 2021

Abstract

Indonesia is the biggest tuna exporter in Southeast Asia. With a high number of tuna catch, it is worried that the catch will decrease tuna population, specifically longtail tuna. To anticipate the decrease, there needs to be a conservation program to protect longtail tunas from scarcity. One method used to protect longtail tuna is by genetic conservation. The aim of this research is to understand the genetic and phylogenetic variety of the longtail tuna in Pabean Surabaya Fish Market. The polymerase chain reaction was used to amplify segment of the mitochondrial control region gene from members of these sample, used forward primer CRK 5’-AGCTC AGCGC CAGAG CGCCG GTCTT GTAAA-3’ and reverse primer CRE 5’-CCTGA AGTAG GAACC AGATG-3’. Based on the sequencing process, 28 out of 29 samples longtail tuna were analyzed successfully. The results of the 28 sample analysis of longtail tuna based on its genetic variety and phylogenetic tree reconstruction showed a haplotype variety (Hd) score of 1,00000, and nucleotide (π) variety of 0,1939. Genetic variety value showed that longtail tuna has great adaption capabilities toward environmental changes time to time. Phylogenetic tree reconstruction results showed 7 clades with a genetic range of 0,005 – 0,035, which shows that all samples are closely related. The results of this study can be used as basic information in forming regulations on longtail tuna sustainable management and genetic conservation.

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Journal Info

Abbrev

fish

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Musamus Fisheries and Marine Journal is a peer-reviewed journal published by faculty of Agriculture Musamus University, Merauke, Indonesia. Musamus Fisheries and Marine Journal published twice a year (October and April) available in electronic and printed version. Hence, we are welcome submission ...