<![CDATA[This study aims to evaluate the effect of C: N ratio level of shrimp feed on inorganic nitrogenregeneration of shrimp feed using molasses as a source of organic carbon. This research wasconducted for two weeks, namely March 8 to March 20, 2021 in the laboratory.Materials and tools used in this study include: plastic containers with a lid and a volume of 2liters of water, aerated stones, aeration sources, sea water and molasses, shrimp feed, waterquality measurement equipment such as thermometers, pH meters, oxygen meters,spectrophotometers and medium for bacterial growth. For the implementation of theexperiment, each experimental container used shrimp feed with a nitrogen content of 5.89%weighing 0.1398 grams. The amount of feed used in each experimental container wasequivalent to 5 mg L-1 ammonia or 4.117 mg L-1 nitrogen. The treatments evaluated in thisexperiment were: (1) Treatment without using molasses (MR0), (2) Treatment using molasseswith a ratio of C: N = 7.5 (MR7.5), (3) Treatment using molasses with a ratio of C: N = 15.0(MR15.0), (4) Treatment using molasses with a ratio of C: N = 17.5 (MR17.5), (5) Treatmentusing molasses with a ratio of C: N = 20.0 (MR20.0) and (6) The treatment used molasses witha ratio of C: N = 22.5 (MR22.5).The experiment used a Completely Randomized Design (CRD)with three replications and the data were analyzed using variance and regression analysis.Furthermore, comparing the average value of each treatment used Duncan's Test. The resultsof measurement of ammonia, nitrite and nitrate concentrations can describe the regeneration ofinorganic nitrogen in shrimp feed in this study. There were a number of results found in thisstudy, including: (1) there was a significant reduction in the regeneration of inorganic nitrogenby increasing the level of C: N ratio in shrimp feed, (2) dissolved oxygen in water decreaseddue to its response to level ratio C: N for shrimp feed, (3) the number of bacteria increases withincreasing levels of the ratio C: N, (4) there is an increase in the number of heterotrophicbacteria by increasing the level of C: N ratio of shrimp feedKey words: Molasses, ratio level, regeneration of inorganic nitrogen, shrimp feed]]>
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