EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Vol 5 No 2 (2020): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi

ISOLASI DAN KARAKTERISASI KEMAMPUAN BAKTERI ENDOFIT SORGUM MANIS FS501 SEBAGAI PENDUKUNG PERTUMBUHAN TANAMAN

Charlie Ester de Fretes (Unknown)



Article Info

Publish Date
31 Dec 2020

Abstract

This study aims to isolate endophytic bacteria of sweet sorghum plants and characterize their ability as plant growth promoting. The characters tested in this study were the ability of endophytic bacteria in N fixation, phosphate dissolving, and IAA production to be developed as biological fertilizer agents. Twenty-four isolates were isolated from the roots, stems and shoots of sweet sorghum. The results of bacterial DNA fingerprint screening showed that 11 groups of endophytic bacteria had different fingerprints. Isolates capable of N fixation were grown on LGI media and showed a change in the color of the medium. The nifH gene detection is also carried out to determine endophytic diazotrophic bacteria. Isolate bacteria that can dissolve inorganic phosphate were tested using Pikovskaya media. Testing the ability of isolates to produce IAA was carried out by adding Salkowski's reagent to the bacterial culture and measured quantitatively with a λ 530 nm spectrophotometer. The results showed that two endophytic bacterial isolates proved to be diazotrophic and three isolates were able to dissolve phosphate, while one isolate was able to produce IAA. PA2 isolate showed ability in all the characters tested, namely N fixation, phosphate solvent and IAA producer.

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Journal Info

Abbrev

ekotonia

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Description

Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi, accept articles in Indonesia and English by covering several topics of biological studies such as botany, zoology, and microbiology. This journal encompasses original research articles, review articles, and short communications, ...