Extraction and Characterization of Collagen from Sand Sea Cucumber (Holothuria scabra)

Gita Syahputra (Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km. 46 Cibinong, Bogor, Indonesia 16911)
Hariyatun Hariyatun (Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km. 46 Cibinong, Bogor, Indonesia 16911)
Muhammad Firdaus (Balai Bio Industri Laut, Indonesian Institute of Sciences (LIPI), Pemenangan, Lombok Utara, Indonesia 83352)
Pugoh Santoso (Biosafety Level 3 and Clean Room Laboratory, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km. 46 Cibinong, Bogor, Indonesia 16911)

Jurnal Ilmu Pertanian Indonesia
Vol. 26 No. 3 (2021): Jurnal Ilmu Pertanian Indonesia

Sand sea cucumber (Holothuria scabra) is an aquatic product that belongs to Echinodermata, a habitant in almost all Indonesian seas. The main component of the sea cucumber is protein, one of which is collagen. This study aimed to extract and characterize collagen from the species using the acid-base extraction method. The characterization of sea cucumber collagen includes molecular weight, amino acid components, Fourier transform infrared spectrophotometry, and scanning electron microscopy analysis. This study has successfully extracted collagen from the sample using an extraction system: NaOH 0.1 M; CH3COOH 0.1 M; and distilled water under 45°C treatments, gave 6% yield. The collagen has a molecular weight 110-130 kDa. Based on the infrared spectra, the specific functional groups of the collagen are amide A (3379.29 cm-1), amide B (2924.09 cm-1), amide I (1681.93 cm-1), amide II (1560.41 cm-1), and amide III (1249.87 cm-1). The collagen falls into type I. We suggest an alternative resource of collagen from sand sea cucumber, other than poultry and mammals. Keywords: characterization, collagen, extraction, fishery, sand sea cucumber