Jurnal Ilmu Pertanian Indonesia
Vol. 26 No. 4 (2021): Jurnal Ilmu Pertanian Indonesia

Penggunaan UV-Vis Spektroskopi dan Kemometrika untuk Uji Keaslian Kopi Codot Lampung

Meinilwita Yulia (Program Studi Mekanisasi Pertanian, Jurusan Teknologi Pertanian, Politeknik Negeri Lampung Jl. Soekarno Hatta No.10, Rajabasa, Bandar Lampung, 35144)
Kurnia Rimadhanti Ningtyas (Program Studi Pengembangan Produk Agroindustri, Jurusan Teknologi Pertanian, Politeknik Negeri Lampung Jl. Soekarno Hatta No.10, Rajabasa, Bandar Lampung, 35144)
Diding Suhandy (Jurusan Teknik Pertanian Universitas Lampung Jl. Prof. Dr. Sumantri Brojonegoro No. 1 Bandar Lampung, 35145)



Article Info

Publish Date
27 Oct 2021

Abstract

Codot coffee from Tanggamus, Lampung is one of Indonesian specialty coffee with a very limited production. In this research, an authentication study for the Codot ground roasted coffee was conducted using UV-vis spectroscopy and chemometrics. A total of 330 samples of pure and adulterated Codot coffee was prepared. The adulterated Codot coffee samples were intentionally created by adding a regular coffee (non-Codot coffee) into pure Codot coffee samples with three levels of adulterations: low (10-20%), medium (30-40%), and high level (50-60%). All samples were 0,29 mm in particle size. The extraction procedure was performed with hot distilled water (98°C). The spectral data of coffee samples were acquired using a benchtop UV-visible spectrometer in the range of 190-1100 nm using a transmittance mode. The result showed that the pure and adulterated samples could be discriminated along PC1 and PC2 axis. The classification model was developed using LDA with 90,91% of accuracy could be obtained. The LDA model was used to classify the new samples and resulted in a sensitivity (SEN) of 100%, specificity (SPEC) of 76,67%, precision (PREC) of 78,13%, and accuracy (ACC) of 87,27% could be obtained. Using PLS regression, a PLS model was developed to quantify the percentages of Codot coffee adulteration and resulted in high of coefficient of determination both in calibration and validation (R2kal = 0,99 and R2val = 0,98). These results showed that UV-vis spectroscopy and chemometrics are suitable for authentication of Codot specialty coffee with RMSEP = 2,68% and RPD in prediction of 6,49. Keywords: authentication, LDA, PCA, PLS regression, UV-vis spectroscopy

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Journal Info

Abbrev

JIPI

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Artikel yang dimuat meliputi hasil-hasil penelitian, analisis kebijakan, dan opini-opini yang berhubungan dengan pertanian dalam arti luas, seperti agronomi, ilmu tanah, hama dan penyakit tanamam, ilmu kehewanan, kedokteran veteriner, keteknikan pertanian, teknologi industri, teknologi pangan, ilmu ...