Pathogenicity of Philippine and Indonesian Trypanosoma evansi Isolates in Mice and Their Responses to Trypanocides

Alan P Dargantes (1College of Veterinary Medicine, Central Mindanao University, Musuan 8710, Bukidnon, Philippines 2School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150, Australia)
April Hari Wardhana (Indonesian Research Center for Veteriner Science)
Jose Alexander C Abella (College of Veterinary Medicine, Central Mindanao University, Musuan 8710, Bukidnon, Philippines)
Milkesidick R Sequito (College of Veterinary Medicine, Central Mindanao University, Musuan 8710, Bukidnon, Philippines)
Simon A Reid (2School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150, Australia)
Douglas Bruce Copeman (Australian Institute of Tropical Veterinary and Animal Science, James Cook University, Townsville 4811, Queensland, Australia)
K AT Dargantes (1College of Veterinary Medicine, Central Mindanao University, Musuan 8710, Bukidnon, Philippines)

Jurnal Ilmu Ternak Veteriner
Vol 26, No 1 (2021): March 2021

Pathogenicity of 10 isolates of T. evansi collected from  Mindanao, Philippines, and one isolate from East Java, Indonesia was determined and compared. The susceptibility of these isolates against diminazene aceturate, melarsomine dihydrochloride, suramin and quinapyramine sulphate/chloride was also tested. Twenty-five mice were infected intraperitoneally with each isolate and 20 were treated with the 4 drugs (5 mice/drug) while 5 infected and 7 uninfected mice served as infected-untreated and uninfected controls, respectively. Treatment was carried out 24 hours post-infection and parasitemia was monitored for 35 days. Mice infected with Philippine isolates significantly died earlier (5-11 days) than those infected with the Indonesian isolate (14-16 days). The prepatent period for Philippine isolates (3-8 days) was significantly shorter than the Indonesian strain (11-13 days).  Trypanosomes were not observed in the blood of mice infected with any of the Philippine isolates when treated with quinapyramine sulphate/chloride, melarsomine dihydrochloride or suramin. Two of 10 mice infected with either C4 or A9 Philippine isolates and treated with diminazene aceturate had parasitemia on days 29 and 31, respectively. It is concluded that isolates of T. evansi from Mindanao, Philippines, are more pathogenic than the isolate from East Java, Indonesia. This study also indicated that quinapyramine sulphate/chloride, melarsomine dihydrochloride and suramin are effective against these T. evansi isolates from Mindanao, Philippines and East Java, Indonesia, while two of the Mindanao isolates are resistant to diminazene. This information is valuable in the enhancement of the control strategy against surra in the Philippines and Indonesia.