Indonesian Journal of Tropical Aquatic
Vol. 4 No. 2 (2021): August

The effectivity of Garlic (Allium sativum L.) and Turmeric (Curcuma longa L.) in Tilapia (Oreochromis niloticus) infected by Edwardsiella tarda

Novi Susanti (Stasiun KIPM Bengkulu)
Dini Oktaviani (Balai KIPM Lampung)
Leni Apriani (Balai KIPM Lampung)
Gustriana Gustriana (Balai KIPM Palembang)



Article Info

Publish Date
28 Feb 2022

Abstract

The aim of this study is to determine the effectivity of Garlic (Allium sativum L.) dan Turmeric (Curcuma longa L.) in Tilapia (Oreochromis niloticus) infected by Edwardsiella tarda. We used sensitivity test with disc diffusion Kirby-Bauer method. The 24 hours purified E. tarda isolate, cultivated in Mueller Hinton Agar (MHA) by swabbing it using a sterile swab on the MHA surface, next put paper disc contain variants garlic concentrations and turmeric. Incubated MHA on the room temperature (30 oC) for at least 24 hours, inhibition zone diameter is measured to see the effectivity. Serial Tube Dilution method is used to determine the Minimum Inhibitory Concentration (MIC) value for garlic and turmeric. Used as medication base on the second stage were 1 × MIC2 × MIC4 × MIC and 8 × MIC. Statistical analysis used completely randomized design, with 6 treatment and 4 repetition which density 10 ind/aquarium each. The result showed that garlic and turmeric inhibited E.tarda effectively, the highest survival rate was 1 × MIC at 8000 ppm for garlic and 1 × MIC at 20 ppm for turmeric. However, MIC level cannot be used as reference to determine the dose of herbal treatment, we can determine the dose by using disc volume in resistance test.

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Journal Info

Abbrev

ijota

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Indonesian Journal of Tropical Aquatic (IJOTA) is open access publishes papers two times a year in February and Augst. IJOTA will publish the research results useful for the sustainability of fisheries. IJOTA is a scientific journal that discusses the results of research in science, technology, ...