Antioxidant activity of red rose leaves (Rosa chinensis jacq.) extract

Deviyanthi Nur Afifah (Department of Pharmacy, Indonesian Health Institute, North Jakarta, Indonesia)
Yessy (Department of Pharmacy, Indonesian Health Institute, North Jakarta, Indonesia)
Farida Nur Rahmani (Department of Pharmacy, Indonesian Health Institute, North Jakarta, Indonesia)
Ayu Lia Ningsih (Department of Pharmacy, Indonesian Health Institute, North Jakarta, Indonesia)
Citra Dewi Puspita Sari (Department of Pharmacy, Indonesian Health Institute, North Jakarta, Indonesia)
Resna Reformasi Yudapradja (Department of Pharmacy, Indonesian Health Institute, North Jakarta, Indonesia)
Praptiwi (Research Center for Chemistry, Indonesian Institute of Sciences, South Tangerang, Banten, Indonesia)
Rahmawati Ridwan (Department of Pharmacy, Indonesian Health Institute, North Jakarta, Indonesia)

Acta Biochimica Indonesiana
Vol. 3 No. 2 (2020): Acta Biochimica Indonesiana

Background: Background: Antioxidants are compounds that can neutralize free radical reactions. The red rose leaves (Rosa chinensis Jacq.), usually disposed of as waste, have been shown to have antioxidant activity. Objective: This study aims to determine the antioxidant activity of red rose leaves extracts. Methods: The red rose leaves were extracted by the maceration method in ethanol, methanol, ethyl acetate, and n-hexane. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and was expressed as IC50 and an antioxidant activity index (AAI). Results: The results showed that the red rose leaves ethanol, methanol, ethyl acetate, and n-hexane extracts had an antioxidant activity with an IC50 value of 1.8, 8.7, 210.3, 48.7 µg/mL, respectively, and an AAI value of 17.3, 3.5, 0.1, 0.6, respectively. The main content of ethanol and methanol extracts are flavonoid, saponin, and tannin. Conclusion: The ethanol and methanol extract showed the most potent antioxidant activity.