Berkala Penelitian Hayati
Vol 3 No 1 (1997): June 1997

ANALISIS 6 DNA REKOMBINAN DENGAN ENZIM EcoR1

Ni Nyoman Tri Puspaningsih (FMIPA Universitas Airlangga)
Akhmaloka (FMIPA Institut Teknologi Bandung)
Afaf Baktir (FMIPA Universitas Airlangga)
Ami Soewandi J.S. (FMIPA Universitas Airlangga)
Y. Sriwulan M. (FMIPA Universitas Airlangga)

Article Info

Publish Date
30 Jan 2014

Abstract

Amylase enzyme from Endomycopsis fibuligera capable to hidrolize starch into glucose. Insertion of amylase gene from Endomycopsis fibuligera into yeast (Saccharomyces cereviceae) will be able to increase the function of yeast (S.cereviceae) to digest more cheaper substrate, like starch. Before cloning in yeast, recombinant DNA will be made and analyzed in Escherichia coli strain DH5a. The result showed that the sixth transformant consist recombinant DNA that were sensitive to tetracycline medium. Analysis by EcoR1 digestion showed that the size of insertion fragment into Ycp 50 vector are around 0.3 untill 16 kb.

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Journal Info
Berkala Penelitian Hayati
Website

Abbrev

jurnal

Publisher

Perhimpunan Biologi Indonesia Cabang Jawa Timur

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Description

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by ...