Oil Recovery Test Using Bio surfactant of Halo tolerant Bacteria Brevundimonas diminuta and Bhurkholderia glumae at variation of NaCl Salt Concentrations

Microbiology Indonesia
Vol. 11 No. 3 (2017): September 2017

Oil recovery test has been done by using crude biosurfactant from Brevundimonas diminuta and Bhurkholderia glumae indigenous halo tolerant bacteria with the vatiation of NaCl salt concentration 0; 1.5; 3; 4.5; 6; and 7.5%. Oil recovery test was obtained by determining % TPH (Total Petrolem Hidrocarbon). The sample concentration was 28.19% TPH, it was extracted by using biosurfactant of  Brevundimonas diminuta and Bhurkholderia glumae bacteria, the optimal salitnity conditions were  at 3, 4.5% salt concentrations with the value oil recovery as much as 50.41, 69.97 % respectively. Oil components which extraction by biosurfactant were analyzed by using GC-MS (Gas Chromatography-Mass Spectrophotometry). The result from analyzes GC-MS could be concluded that bacteria Brevundimonas diminuta could dissolve hydrocarbon compounds short chain carbon atom at fraction <C10–C14 and long chain carbon atom at fraction >C22. <C10, C11-C14dan C15-C17  and Bhurkholderia glumae could dissolve hydrocarbon compounds short chain carbon atom at fraction <C10–C14 and long chain carbon atom at fraction >C22 according to the retention time.