BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Vol. 23 No. 1 (2016)

CELLULASE PRODUCTION BY Bacillus subtilis M1 USING PRETREATED GROUNDNUT SHELL BASED LIQUID STATE FERMENTATION

Ashish Kr Vyas (LPU PHAGWARA)
Chayanika Putatunda (Unknown)
Joginder Singh (Unknown)
Deepak Vyas (Unknown)

Article Info

Publish Date
03 Aug 2016

Abstract

Groundnut shell which is rich in natural cellulose was assessed as a substrate for production of cellulase enzyme by cellulolytic bacteria. In the present investigation the bacterial isolate Bacillus subtilis M1 was found to be capable of producing high amount of endoglucanase and exoglucanase on alkali treated groundnut shell. The effect of some nitrogen sources, amino acids and Ca++ ionsin the medium containing pretreated groundnut shell were also evaluated. It was observed that 2% substrate concentration, 1mM calcium concentration were optimum for cellulase production. Ammonium nitrate was found to be the best among nitrogen sources tested. Asparagine, tryptophan and methionine were found to be stimulatory for cellulase activity.Keywords: Bacillus subtilis, cellulase, endoglucanase, exoglucanase, groundnut shell

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Journal Info
BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Website

Abbrev

biotropia

Publisher

Southeast Asian Regional Centre for Tropical Biology

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Description

BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and ...