BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Vol. 23 No. 1 (2016): BIOTROPIA Vol. 23 No. 1 June 2016

Cellulase Production by Bacillus subtilis M1 Using Pretreated Groundnut Shell Based Liquid State Fermentation

Vyas, Ashish Kr (Unknown)
Putatunda, Chayanika (Unknown)
Singh, Joginder (Unknown)
Vyas, Deepak (Unknown)

Article Info

Publish Date
03 Aug 2016

Abstract

Groundnut shell, which is rich in natural cellulose, was assessed as a substrate for the production of cellulase enzyme by cellulolytic bacteria. In the present investigation, the bacterial isolate M1 was found to be capable of producing high amounts of endoglucanase and exoglucanase on alkali-treated groundnut shell. The effects of various nitrogen sources, amino acids, and Ca²⁺ ions in the medium containing pretreated groundnut shell were also evaluated. It was observed that a 2% substrate concentration and 1 mM calcium concentration were optimal for cellulase production. Among the nitrogen sources tested, ammonium nitrate was found to be the most effective. Asparagine, tryptophan, and methionine were found to stimulate cellulase activity.

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Journal Info
BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Website

Abbrev

biotropia

Publisher

Southeast Asian Regional Centre for Tropical Biology

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Description

BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and ...