The enamel thickness of gestational diabetes mellitus rat offspring

Nurdiana Dewi (Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Lambung Mangkurat, Banjarmasin, Indonesia)
Ahmad Syaify (Department of Periodontology, Faculty of Dentistry, Universitas Gadjah Mada Yogyakarta, Indonesia)
Ivan A. Wahyudi (Department of Biomedica, Faculty of Dentistry, Universitas Gadjah Mada Yogyakarta, Indonesia)

Journal of Dentomaxillofacial Science
Vol. 6 No. 3 (2021): (Available online: 1 December 2021)

Objective: To determine the molar enamel thickness of rat offsprings born to diabetic rat.Material and Methods: This research used sixteen rat offsprings born to 8 diabetic rats who had been injected with STZ dose of 40 mg / kg and 16 rat offsprings born to 8 control rats. Rat offsprings were decapitated on day 5 after birth and taken right mandibular molar tooth germ. Tooth germ then embedded in paraffin block. Haematoxylin eosin staining performed for histological analysis. Enamel thickness measured on the images using ImageJ Software at 5 field of view.Results: The enamel thickness on the Diabetes Mellitus (DM) group (333.02 ± 135.81 µm) were lower than control group (415.39 ± 146.17 µm). Mann Whitney test results showed a significant difference between the DM and control group.Conclusion: It was concluded that enamel thickness decreases in gestational diabetes mellitus rat offspring.