Jurnal ULTIMATICS
Vol 13 No 2 (2021): Ultimatics : Jurnal Teknik Informatika

Classification of Metagenome Fragments With Agglomerative Hierarchical Clustering

Alex Kurniadi (Universitas Multimedia Nusantara)
Marlinda Vasty Overbeek (Universitas Multimedia Nusantara)



Article Info

Publish Date
23 Jan 2022

Abstract

Unlike genomics which study specifically culturable microorganisms, metagenomics is a field that studies microorganic samples retrieved directly from the environment. Such samples produce widely varying fragments when sequenced, many of which are still unidentified or unknown. Assembly of these fragments in the goals of identifying the species contained among them are thus prone to make said goals more difficult, so it becomes necessary for binning techniques to come in handy while trying to classify these mixed fragments onto certain levels in the phylogenetic tree. This research attempts to implement algorithms and methods such as k-mers to use for feature extraction, linear discriminant analysis (LDA) for dimensionality reduction, and agglomerative hierarchical clustering (AGNES) for taxonomic classification to the genus level. Experimentation is done across different objective measurements, including the length of the observed metagenome fragment that spans from 0,5 Kbp up to 10 Kbp for both the 3-mer and 4-mer contexts (k = 3 and k = 4). The averaged validity scores of the resulting data clusters generated from both the training and test sets, computed with the silhouette index metric, are 0.6945 and 0.0879 for the 3-mer context, along with 0.5219 and 0.1884 for the 4-mer context.

Copyrights © 2021






Journal Info

Abbrev

TI

Publisher

Subject

Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Engineering

Description

Jurnal ULTIMATICS merupakan Jurnal Program Studi Teknik Informatika Universitas Multimedia Nusantara yang menyajikan artikel-artikel penelitian ilmiah dalam bidang analisis dan desain sistem, programming, algoritma, rekayasa perangkat lunak, serta isu-isu teoritis dan praktis yang terkini, mencakup ...