Effectiveness of class 1 biological safety cabinet modification on reducing air germ rate

Budi Setiawan (Pusat Unggulan Iptek Inovasi Teknologi Terapan Kesehatan Masyarakat (PUINOVAKESMAS), Poltekkes Kemenkes Yogyakarta, Indonesia)
Befani Adhitya Febriana (Department of Medical Laboratory Technology, Poltekkes Kemenkes Manado, Indonesia)
Budi Martono (Department of Medical Laboratory Technology, Poltekkes Kemenkes Yogyakarta, Indonesia)
Saptono Putro (Department of Medical Laboratory Technology, Poltekkes Kemenkes Yogyakarta, Indonesia)

PUINOVAKESMAS
Vol. 2 No. 1 (2021): April

Biological Safety Cabinet (BSC) in the form of a ventilated cupboard using a combination of High-Efficiency Particulate Air (HEPA) filtration system, laminar airflow, and containment to protect laboratory, product, and the environment from harmful biological agents. This research aims to determine the effect of level 1 BSC created to decrease the number of air germs in the laboratory. This study was an observational analytic using a cohort design. The research object is the number of air germs in standard Level 1 BSC Safe-tech ES 199 models and level 1 BSC created from a team in the laboratory. Data analysis using one-way ANOVA and independent t-test. One-way ANOVA test data shows the standard level 1 BSC Safe-tech ES 199 models and the creation of level 1 BSC used in 60 minutes and 90 minutes is the same as significant (p>0.05), while the duration of use for 120 minutes has a significant difference (p<0.05). Independent t-test analysis showed Sig (2-tailed) = 0.577, so there were no differences between the two tools during the 120-minute use. The creation of level 1 BSC can reduce the number of air germs.