Jurnal Sain Peternakan Indonesia
Vol 16, No 4 (2021)

Effect Concentration of Moringa (Moringa oleifera Lam) Leaf Extract in Citrate-Egg Yolk in Maintaining Motility and Viability of Spermatozoa of Kacang Goat

R. R. Dapawole (Universitas Pertahanan Republik Indonesia)
I. P. Sirappa (Universitas Kristen Wira Wacana Sumba)



Article Info

Publish Date
26 Dec 2021

Abstract

This study aimed to determine the effectiveness and the best concentration of Moringa leaf extract (MLE) in the citrate-egg yolk (C-EY) to maintain the motility and viability of spermatozoa kacang goat. Semen was collected from 3 goats aged two years; by using the artificial vagina method. The semen was evaluated macroscopically and microscopically. The semen that had >70% sperm motility and >250x106/ml sperm concentration was divided into 4 equal tubes, each diluted with100% C-EY (P1), 10% MLE+ 90%C-EY (P2), 20% MLE +80% C-EY (P3), and 30% MLE+70% C-EY (P4). The diluted samples were then stored in a refrigerator (3-5?C) and evaluated for motility and viability every 24 hours. The study was designed using a completely randomized design (CRD) consisting of four treatments and five replications. The results showed that the addition of MLE in C-EY significantly affected goat spermatozoa's progressive motility and viability. The data showed that the spermatozoa kept during four days in a diluent of P2 had higher (P<0.05) motility 44.67±4.80% and viability 74.24±4.46%than the other three diluents of P1(36.00±4.70%; 70.10±3.6%), P3(33.67±0.42%; 66.85±4.99%) and P4 (29.67±3.99%; 63.96±5.44%). This study concluded that adding 10% MLE was the best concentration as source energy in 90% C-EY diluents, which effectively maintained the motility and viability of kacang goat spermatozoa for four days of storage at a temperature of 3-5oC.

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Journal Info

Abbrev

jspi

Publisher

Subject

Veterinary

Description

Jurnal Sain Peternakan Indonesia (JSPI) pISSN 1978 – 3000 dan eISSN 2528 – 7109 adalah majalah ilmiah resmi yang dikeluarkan oleh Jurusan Peternakan Fakultas Pertanian Universitas Bengkulu, sebagai sumbangannya kepada pengembangan Ilmu Peternakan yang diterbitkan dalam Bahasa Indonesia ...