Lymphocyte Proliferation Activity MTT -Test of Ethanolic Extract of Pasak Bumi Root (Eurycoma longifolia Jack) on Induced 7,12-Dimethylbenz[A] Antracene (DMBA) Female Sprague Dawley Rat

Falah, Lily Noor ( Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta)
Yuliani, Sapto ( Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta)

Indonesian Journal of Cancer Chemoprevention
Vol 2, No 1 (2011)

Pasak bumi root (Eurycoma longifolia Jack) contains quasinoid. Quasinoid potentially has  antitumor/anticancer,  anti-parasitic,  and  immunostimulatory  activity.  The  aim  of  this research was to study the lymphocyte proliferation  activity MTT-test   of ethanol extract of  Eurycoma  longifolia  Jack  root  on  induced  by  7,12-dimethylbenz[a]  antracene  (DMBA) female Sprague Dawley rat. The test was done at 6 groups of 10  SD rat each. Each groups was  administered  orally  with  DMBA  (Group  I)    dose  of  20 mg/kg  bw,  corn oil  (Group  II), baseline (Group III), and  ethanol extract of pasak bumi root dose 12.6; 25.2; and 50.4 mg/kg bw (Group IV,V,and VI). Ethanol extract is given for 5 weeks. At the second week five rats were  sacrificed  to  isolate  the  lymphocytes  of  spleen  then  rats  are  injected  orally  with DMBA 2 times a week for 5 weeks. At the eight until twenty fourth week all groups were just  administered  by  aquades.  Measurement  of  spleen  relative  weight  and  isolation  of  the lymphocyte  spleen  were  done  at  second  week  and  the  last  of  experiment.  Lymphocyte proliferation  activity  were  measured  by  MTT-reduction  method.  Data  were  analyzed statistically  by  analyzes  of  variance  (anova)  continued  by      LSD  test  and  Kruskall  Wallis continued by Mann Whitney test. The result showed that limphocyte proliferation activity MTT of ethanol extract of E. longifolia root doses of 12.6; 25.2; and 50.4 mg/kg bw for 14 days prior to DMBA-induced are  339.35 ± 8.43;61.20 ± 6.27;  310.28 ±  6.81   and  dose 50,4 mg/kg bw after  DMBA-induced is 122.108 ±  11.426