Pengaruh Triptofan pada Pertumbuhan dan Kandungan Katarantin dari Kalus Catharanthus roseus

Dingse Pandiangan ( Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sam Ratulangi)
Dennie Heroike Rompas ( Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Manado)
Henry Fonda Aritonang ( Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sam Ratulangi)
Rizkita Rachmi Esyanti ( Program Studi Biologi, Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung)
Erly Marwan ( Program Studi Biologi, Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung)

Jurnal Matematika & Sains
Vol 11, No 4 (2006)

Research on the growth and catharanthine content of Catharanthus roseus callus which were treated with tryptophan had been done. Wet and dry weight of Catharanthus roseus callus decreased when treated with 100, 125 and 225 mg/L tryptophan, but callus treated with 150, 175 and 200 mg/L tryptophan grew better. Catharanthine contents of the kontrol callus was 423,22 µg/g dw. The treatment calli contains 485,00, 588,32, 875,10, 905,26, 784,10 and 950,54 µg/g dw of catharanthine respectively. The optimal tryptophan treatment for callus growth and catharanthine production was 175 mg/L tryptophan with a 113,90 %. increase of catharanthine content.